FileBuzz: Software Download
Find shareware, freeware downloads from thousands of software titles

Program Name: PerlPrimer

Published By: Owen Marshall

License Type: Shareware

Date Released: January 20, 2011


PerlPrimer v1.1 Instant Download

PerlPrimer Desciption:


Advertisements



PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard PCR, bisulphite PCR, real-time PCR (QPCR) and sequencing. It aims to automate and simplify the process of primer design. PerlPrimer's current features include the following: - Calculation of possible primer-dimers - Retrieval of genomic or cdna sequences from Ensembl (including both sequences automatically for QPCR) - Ability to BLAST search primers using the NCBI server or a local server - Results can be saved or optionally exported in a tab-delimited format that is compatible with most spreadsheet applications. - ORF and CpG island detection algorithms - Ability to add cloning sequences to primers, automatically adjusted to be in-frame - QPCR primer design without manual intron-exon boundary entry PerlPrimer calculates primer melting temperature using J. SantaLucia's extensive nearest-neighbour thermodynamic parameters. To adjust for the salt conditions of the PCR, PerlPrimer uses the empirical formula derived by von Ahsen, et al. (2001) and allows the user to specify the concentration of Mg2 , dNTPs and primers, or use standard PCR conditions. The result is a highly accurate prediction of primer melting temperature, giving rise to a maximum yeild of product when amplified.




License: Shareware | Price: $0.00 | Size: 3.3 MB | Downloads (152)


Related Software
New Reviews
New Downloads Top Downloads Top Search

New Downloads

SentiVeillance SDK Trial
MinimizeToTrayTool
Mytoolsoft Watermark Software
Elcomsoft Phone Viewer
SmartVizor Variable Barcode Label Printing Software
ConyEdit for Windows
Question Tools Editor
Yasisoft GIF Animator
IHDP InHouse Digital Publishing Win/Mac
SmartVizor Bill Statement Batch Printing Software
FlexiHub
Network Password Manager
Atlantis Word Processor
Checksum Aide 64 bit
Checksum Aide
C Pivot for Mac
C Pivot
My SCUBA Diary
DataNumen Word Repair
Handy Backup Standard
DiskGenius Free
Altova UModel Enterprise Edition
Emsisoft Anti-Malware
Atlantis Word Processor Lite
DiskGenius Standard

Top Downloads

Opera Mini
Turbo C++
Abyss Web Server X1
TaskMerlin Project Management Software
Macrorit Disk Partition Expert Server Edition
Foxit Advanced PDF Editor
Kids Online Browser
Rapid PHP Editor
MathCast
Cleanup and Update Tool for Cisco CUCM
FTP!
Open-School Community Edition
Count Code
simplitec simplisafe
Multi-Process Killer Portable
SCEA Part 2 & 3 Exam EPractize Labs Enterprise
Silva 2.1a2
Agama Web Menus
aXmag Free
Flash Player Pro
Red Call Recorder
Syston Data Recovery Free
Photo! 3D Album
Photo! Editor
Sondle Virtual Desktop Assist

Top Search

Free Oxford English To Tamil Dictionary
Zawgyi Myanmar Font For Android Mobile
Opera Mini
Ucbroser Download High Speed Of Internet
Free Tecno Phone Whatsapp Messenger
Free Photofunia Full Version
Adobe Flash Player
New Action Plus Voip
Ifone Platinum Pc To Phone
Uc Browser
Bolt Browser Free Download For Pc
Free Hindi To English Translation
Jumbo Ring Call Software
Free Mobile Antivirus
Free English To Marathi Translation
Samsung Intelli Studio
Purble Place Game Free Download
Download Action Voip Plus Free Download
Mobeely Phone Dailer
Free Mobile Zenga Tv
Free Cell Phone Tracking Software
Ucwap Browser
Free Call Recorder For Samsung Mobile
Purble Place
Nimbas Phone Soft Ware